FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_17_tga1_wt.3510000003d21e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_17_tga1_wt.3510000003d21e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13112896
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC827790.6312793146533001TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG584080.4454241076875772No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG461120.35165382231354536No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA308310.23511968675721975No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC307900.23480701745823349No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG255240.19464807773965415No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC225720.17213588821264197No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT218900.1669349013368214No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT160260.12221556550132022No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA159590.12170461811029387No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT159120.1213461923285291No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG147790.11270584316385944No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA145570.11101285330105569No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT141960.1082598382538838No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG140510.1071540565867372No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC136360.10398923319455901No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA135310.10318849474593561No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC133440.10176241769933965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG103600.040.91752211
GTATGCC100300.040.8031345
CGTATGC101450.040.2956344
ACGTCTG105950.039.79926715
AGCACAC107650.039.16702710
CTCGTAT104550.039.1223542
TCGTATG105650.038.7150243
CACACGT109650.038.57779712
CGTCTGA110600.038.18700416
ACACGTC110900.038.16442513
CACGTCT111050.038.0723514
GAGCACA111300.037.7806329
TCTCGTA110400.037.09033241
CATCTCG110650.036.72186739
CAGTCAC112050.036.52686327
AGAGCAC116800.036.0593648
AGTCACC113600.035.85048728
CTGAACT118950.035.42840619
CCAGTCA119000.034.56374426
TCCAGTC122500.034.4190225
Produced by FastQC (version 0.11.2)