FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_15_tga1_wt.3510000003d139.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_15_tga1_wt.3510000003d139.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15067900
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG715310.47472441415193883No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC661440.43897291593387266TruSeq Adapter, Index 5 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG550510.36535283616164166No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA377220.25034676364987823No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC359060.23829465287133572No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG345480.22928211628694112No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT258940.17184876459227896No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC243430.16155535940642027No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA235740.15645179487519825No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA196500.13040967885372215No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT192220.12756920340591588No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT188750.12526629457323182No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC182890.12137723239469336No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT173360.11505252888590978No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG171870.11406367177908003No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC171270.11366547428639691No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG171020.11349955866444561No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA152870.10145408451078121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG87200.038.83209611
CGTATGC85400.038.62167444
GTATGCC85850.038.49861545
ACGTCTG88250.038.09253315
AGCACAC92700.036.57595410
CACACGT92850.036.5177212
CTCGTAT90250.036.37189542
TCGTATG91050.036.10149843
ACACGTC93550.036.07731213
CGTCTGA94250.035.54817216
CACGTCT95150.035.49429714
ACACAGT94050.034.88187432
GTCACAC95600.034.4800529
CACAGTG95550.034.3099333
CACACAG96450.034.03722431
CAGTCAC96550.033.97754327
GAGCACA100300.033.893559
AGAGCAC102100.033.3400848
TCTCGTA98900.033.07731641
CTGAACT103050.032.5554419
Produced by FastQC (version 0.11.2)