FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_14_tga1_wt.3510000003d0e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_14_tga1_wt.3510000003d0e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16248965
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG782500.48156913378790583No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG579260.3564903980038113No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC451360.2777776923022482TruSeq Adapter, Index 4 (100% over 51bp)
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA395140.24317856552709666No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG391560.24097534827602868No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC379700.2336764218520995No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT268380.16516744297252162No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA243550.14988646969206962No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC239980.1476894066791331No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT200920.1236509525375924No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC195140.12009380289759995No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA189470.1166043498770537No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT184730.11368724100273464No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT182490.11230869166128427No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC179910.11072089822336377No Hit
CTGGAACTGAGGGGACAGGATGTCCCAGGCGAAGGGCAGGGGGCCGCCCTT164120.10100335621376501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG62750.036.68085511
GTATGCC61400.036.1306245
CGTATGC62850.035.36745544
ACGTCTG66700.034.4095215
AGCACAC66600.034.289310
CACACGT67900.033.79942312
ACACGTC67800.033.651413
ACTGACC66800.033.1797732
CTCGTAT67500.032.66438342
TCGTATG68450.032.47398843
CACGTCT71650.032.0316214
CGTCTGA73300.031.40334316
GAGCACA73400.031.3261479
CACTGAC72400.030.7687831
AGAGCAC76200.030.027438
AGTCACT75050.029.4113228
TCTCGTA76750.029.0207741
CAGTCAC76000.029.01389527
CTGAACT81850.027.90175819
CTGACCA80600.027.69369333
Produced by FastQC (version 0.11.2)