Basic Statistics
Measure | Value |
---|---|
Filename | C7KGBACXX_l04n01_13_tga1_wt.3510000003d08a.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20239548 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC | 97781 | 0.48311849651978395 | TruSeq Adapter, Index 3 (100% over 51bp) |
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG | 90304 | 0.4461759719139973 | No Hit |
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG | 65711 | 0.32466634136295924 | No Hit |
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC | 47132 | 0.23287081312290178 | No Hit |
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG | 47129 | 0.23285599065749887 | No Hit |
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA | 43639 | 0.2156125225721444 | No Hit |
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT | 30101 | 0.1487236770307321 | No Hit |
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA | 27258 | 0.1346769206505995 | No Hit |
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC | 26442 | 0.1306452100610152 | No Hit |
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT | 24575 | 0.12142069575862069 | No Hit |
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT | 22551 | 0.11142047243347529 | No Hit |
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC | 22384 | 0.1105953551927148 | No Hit |
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA | 21431 | 0.10588675201639879 | No Hit |
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA | 21024 | 0.10387583754340758 | No Hit |
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC | 20644 | 0.10199832525904236 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCACACG | 11940 | 0.0 | 40.11983 | 11 |
CGTATGC | 11725 | 0.0 | 39.66249 | 44 |
GTATGCC | 11740 | 0.0 | 39.459282 | 45 |
ACGTCTG | 12345 | 0.0 | 38.67824 | 15 |
TAGGCAT | 12060 | 0.0 | 38.17248 | 35 |
AGCACAC | 12580 | 0.0 | 37.95159 | 10 |
ACTTAGG | 12285 | 0.0 | 37.822372 | 32 |
CACTTAG | 12420 | 0.0 | 37.375027 | 31 |
CTCGTAT | 12525 | 0.0 | 37.039436 | 42 |
TCGTATG | 12555 | 0.0 | 37.02252 | 43 |
CACACGT | 12950 | 0.0 | 37.008167 | 12 |
TTAGGCA | 12610 | 0.0 | 36.614697 | 34 |
ACACGTC | 13130 | 0.0 | 36.382034 | 13 |
CACGTCT | 13175 | 0.0 | 36.34316 | 14 |
CGTCTGA | 13355 | 0.0 | 35.88791 | 16 |
CATCTCG | 13135 | 0.0 | 35.285297 | 39 |
AGGCATC | 13110 | 0.0 | 35.131134 | 36 |
GAGCACA | 13700 | 0.0 | 35.07777 | 9 |
TCACTTA | 13315 | 0.0 | 35.048058 | 30 |
GGCATCT | 13420 | 0.0 | 34.403355 | 37 |