FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_13_tga1_wt.3510000003d08a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_13_tga1_wt.3510000003d08a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20239548
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC977810.48311849651978395TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG903040.4461759719139973No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG657110.32466634136295924No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC471320.23287081312290178No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG471290.23285599065749887No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA436390.2156125225721444No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT301010.1487236770307321No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA272580.1346769206505995No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC264420.1306452100610152No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT245750.12142069575862069No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT225510.11142047243347529No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC223840.1105953551927148No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA214310.10588675201639879No Hit
TCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGA210240.10387583754340758No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC206440.10199832525904236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG119400.040.1198311
CGTATGC117250.039.6624944
GTATGCC117400.039.45928245
ACGTCTG123450.038.6782415
TAGGCAT120600.038.1724835
AGCACAC125800.037.9515910
ACTTAGG122850.037.82237232
CACTTAG124200.037.37502731
CTCGTAT125250.037.03943642
TCGTATG125550.037.0225243
CACACGT129500.037.00816712
TTAGGCA126100.036.61469734
ACACGTC131300.036.38203413
CACGTCT131750.036.3431614
CGTCTGA133550.035.8879116
CATCTCG131350.035.28529739
AGGCATC131100.035.13113436
GAGCACA137000.035.077779
TCACTTA133150.035.04805830
GGCATCT134200.034.40335537