FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_12_tga1_wt.3510000003d03a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_12_tga1_wt.3510000003d03a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17171161
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG647990.3773711049590648No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC613800.35745981299691965TruSeq Adapter, Index 2 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG499340.2908015363667023No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG369530.21520385255254437No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA362820.21129613775096512No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC350710.20424361521041004No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT252290.14692658230855793No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA244760.1425413226280972No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC226300.13179073913522796No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT212020.12347446978104742No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT209430.12196612680994605No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG201340.11725473891951745No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA193680.11279377090459987No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG191700.11164067473364207No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC190410.11088941510710895No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC189010.11007409458218928No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT188420.109730495218116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG80400.039.06614311
CGTATGC80200.037.7588844
GTATGCC80250.037.65345
AGCACAC86500.036.41406610
ACGTCTG86950.036.2037415
CACACGT88100.035.77953312
ACACGTC88750.035.31600613
CACGTCT90450.034.8761314
TATCTCG86800.034.8104639
CTCGTAT87600.034.72329742
TCGTATG87500.034.68584443
CGATGTA88050.034.16624534
CGTCTGA94750.033.29462416
GAGCACA96300.032.8010679
AGTCACC93850.032.5822928
CAGTCAC93800.032.5754827
CCGATGT94350.032.43356733
AGAGCAC98250.032.195858
TCTCGTA96050.031.62213141
ACCGATG100850.030.25452632