FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_11_tga1_wt.3510000003cfde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_11_tga1_wt.3510000003cfde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16590804
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG652620.39336249165501563No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG491190.29606160135458176No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG382710.23067598170649234No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA367380.22143592317768324No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC365350.2202123537834574No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC225230.13575592840467526No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA224120.13508688307088673No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT223690.13482770334698668No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT209430.12623258041020796No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC196900.11868020380446903No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT195760.11799307616436189No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG186610.11247797273718622No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC172860.10419024900782385No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT172820.10416613926606572No Hit
CCGACATCAAGCTGGACATCACCTCCCACAACGAGGACTACACCATCGTGG171990.1036658621245842No Hit
CCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA171960.10364777981826559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT79150.024.593611
ACTCTAG99250.019.9961075
CTGACTC98150.019.8318522
CTTCCGA67950.019.4404261
GACTCTA102100.019.3731064
CTAGCAG108450.017.8825248
TGACTCT113050.017.5762523
CTCTAGC118100.016.6119376
GCACACG14650.016.12647211
TAGCAGA122150.015.9321259
CCGATCT81250.015.8419564
TTCCGAT87400.015.1392162
CTATCGA134300.014.4920717
TCTATCG135100.014.27353416
CCGGGGT9500.014.2129671
CGTATGC14250.014.05161544
GCAGATC148800.013.23100611
ACGTCTG18850.013.13068515
GATCTAT149050.013.01288214
CGCGGGG8150.012.9776891
Produced by FastQC (version 0.11.2)