FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_112_tga1_wt.3510000003d3a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_112_tga1_wt.3510000003d3a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14058847
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC2488031.769725497403877TruSeq Adapter, Index 12 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG403220.2868087262063525No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG278390.19801766105001353No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA249700.17761058214802394No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249270.17730472491805338No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC208250.1481273677706287No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC155370.11051404144308563No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG154750.11007303799522108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140680.10006510491223071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC285500.042.4612445
CGTATGC289700.041.82100744
CTCGTAT290950.041.50228542
TCGTATG293200.041.26806343
GCACACG309550.040.44979511
TCTCGTA301700.040.03124241
ACGTCTG313150.039.92900515
CAGTCAC305650.039.83199327
CACGTCT315100.039.70219414
CACACGT315350.039.69869612
CCAGTCA306650.039.63606326
AGCACAC315200.039.61652810
ACACGTC315350.039.5922413
AGTCACC305750.039.5103128
CGTCTGA318600.039.27422716
TGTAATC309200.039.039836
TCCAGTC319450.038.8567625
GTAATCT311000.038.74149737
CTGAACT322550.038.7286919
ATCTCGT311650.038.6593240