FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_111_tga1_wt.3510000003d351.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_111_tga1_wt.3510000003d351.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11378702
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG291310.25601338360034387No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG206510.18148818731697167No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA203830.17913290988726133No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC145470.12784410735073298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG22350.026.17391811
GTATGCC21250.025.72861745
CGTATGC22100.024.73807744
CGGCTAC23250.024.19439533
ACGGCTA24250.022.91873232
ACGTCTG26700.022.16389315
AGTCACG25000.022.14077628
AGCACAC27250.021.71454410
CTCGTAT25850.021.41048642
CACACGT28200.021.22293512
TCGTATG26300.021.1296943
CACGGCT26300.020.9611831
ACACGTC28800.020.31267713
CACGTCT29150.020.30034814
GGCTACA27550.019.92792934
GCTACAT28700.019.83501635
CGTCTGA31350.019.01997416
AGAGCAC32750.018.2734418
CTACATC30550.018.26465636
GAGCACA33000.018.1350069
Produced by FastQC (version 0.11.2)