FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l04n01_110_tga1_wt.3510000003d2e5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l04n01_110_tga1_wt.3510000003d2e5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13531087
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG366130.2705843218656417No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA262010.19363558892201344No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG245750.18161881599017138No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC183930.13593142960354923No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC162030.11974647713077302No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC145660.10764840991710423TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26700.028.3125244
GTATGCC26900.028.10284845
GCACACG31050.026.52125511
ACGTCTG32650.025.08506815
CTCGTAT30450.024.67798442
TATCTCG31500.024.28428339
TCGTATG31200.023.94054443
ACACGTC36150.022.84258313
AGCACAC36400.022.68427310
CACTAGC33600.022.63560731
CACACGT36400.022.62321512
TAGCTTA33350.022.60262534
CACGTCT36400.022.50025414
CGTCTGA38150.021.58656716
ACTAGCT35450.021.26391832
TCACTAG36250.021.16661830
GTCACTA37100.020.8635429
TCTCGTA39000.019.6142341
AGAGCAC44650.019.0971378
CAGTCAC40900.019.09009627
Produced by FastQC (version 0.11.2)