FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_9.3510000003d1f3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_9.3510000003d1f3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17018183
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC887590.521553916772431TruSeq Adapter, Index 9 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG363090.21335415185040613No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG310730.18258705996991573No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG260700.15318909192597116No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT217960.12807477743070456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG109650.040.48179611
GTATGCC109800.039.89692345
CGTATGC110650.039.6915344
ACGTCTG114800.038.7088215
AGCACAC117100.037.8102510
AGTCACG116200.037.7562628
CTCGTAT116050.037.68984642
GTCACGA116750.037.38590229
CACACGT119750.037.08625412
ACACGTC119900.037.00428413
CGTCTGA122950.036.1613316
CACGTCT124000.035.89057514
TCTCGTA124400.035.27040541
TCACGAT124400.035.2315430
GAGCACA126450.035.0855379
CAGTCAC126350.034.63417427
AGAGCAC128850.034.4145628
CACGATC129150.033.8497531
TCGTATG130200.033.83538443
CTGAACT132300.033.6231119