FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_8.3510000003d1cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_8.3510000003d1cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20167255
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1100610.5457411035859863TruSeq Adapter, Index 8 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG639650.31717256513095116No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG391500.19412656804309758No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA387100.19194481351081247No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC303980.15072948698273514No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG290940.14426355991432646No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT227190.11265291186133164No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA216500.10735224005448435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC133100.040.0624345
GCACACG136100.039.987711
CGTATGC133750.039.80005344
ACGTCTG144100.037.80389815
AGCACAC144750.037.73799510
CTCGTAT141050.037.67678542
ACACGTC147700.036.97085613
CACACGT148450.036.75193812
CACGTCT152500.035.73536714
CGTCTGA153750.035.47516316
GAGCACA155100.035.248539
AGAGCAC154900.035.1778458
GTCACAC152500.035.11350329
TCGTATG154200.034.59472743
CAGTCAC154300.034.49931327
TCTCGTA155450.034.27456741
CTGAACT162750.033.45834419
AGTCACA163650.032.45972428
CCAGTCA166750.032.099326
TCGGAAG170400.032.0882263