FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_7.3510000003d16f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_7.3510000003d16f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19891854
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1217430.6120243995356089TruSeq Adapter, Index 7 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG636620.3200405552946447No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA386380.19424031565886218No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG376910.1894795728945125No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC289480.14552690764772352No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG281910.14172132974633736No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA221290.11124654343431235No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT204810.1029617450439763No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC152800.040.56672345
CGTATGC155650.039.80877744
GCACACG158900.039.74320611
CTCGTAT160750.038.47590642
ACGTCTG165600.038.08568215
AGCACAC166050.037.9911510
CACACGT168150.037.4498812
ACACGTC170800.036.92356513
CACGTCT175500.035.92371714
GAGCACA176300.035.884459
CATCTCG172700.035.8818639
CGTCTGA176050.035.8508216
TCTCGTA173750.035.65054741
TCGTATG174150.035.63155743
CAGTCAC173950.035.53060527
AGAGCAC178200.035.4765938
CTGAACT184500.034.1844819
AGTCACC180150.034.08324428
CCAGTCA186850.033.09005426
TCGGAAG194600.032.434283