FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_6.3510000003d11f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_6.3510000003d11f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16852804
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC724470.42988098597717034TruSeq Adapter, Index 6 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG591890.3512115847309445No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG375150.2226039061511663No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA371090.22019481149843076No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC281980.16731933748235606No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG248480.14744133973195203No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT211920.1257476203959887No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA202170.11996223299102038No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT175720.10426751536420882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG93850.038.64283811
GTATGCC93450.037.82354745
CGTATGC94200.037.6888144
TCACGCC93750.037.60651430
ACGTCTG100450.036.10769315
AGCACAC100550.036.0455610
CACGCCA97950.035.9039131
GTCACGC99000.035.7713129
CTCGTAT99500.035.6363642
CACACGT102150.035.4809712
AGTCACG100200.035.34259828
ACACGTC104200.034.8062713
CGTCTGA107550.033.76605616
CGCCAAT104400.033.75136633
CACGTCT107550.033.74433514
TATCTCG104950.033.7252739
GCCAATA104700.033.37775434
ACGCCAA106350.033.0680632
AGAGCAC110050.032.9134068
GAGCACA111900.032.711059