FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_5.3510000003d0b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_5.3510000003d0b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16484528
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG600620.3643537746425011No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC467720.28373272198027144TruSeq Adapter, Index 5 (100% over 51bp)
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG358470.2174584555893866No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA322380.1955651990763703No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC265790.1612360390300529No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG247700.15026211244871554No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT188600.1144103125063696No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA165710.10052456460991786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG65200.036.43845711
GTATGCC64650.035.4625945
CGTATGC65400.034.9177744
ACGTCTG71150.033.42611715
AGCACAC71000.033.3982210
CTCGTAT69350.033.1565442
ACACGTC72900.032.6838513
CACACGT72550.032.6538712
CACAGTG73150.031.09852633
ACACAGT73600.030.78488232
CGTCTGA78050.030.50000616
GAGCACA78550.030.2449049
GTCACAC75800.029.97971529
CACACAG76950.029.85412431
CACGTCT80150.029.81289914
AGAGCAC82650.028.9350938
CAGTCAC79600.028.40667527
TCGTATG80650.028.28723143
TCTCGTA81500.028.13254541
CTGAACT84850.027.87040519