FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_4.3510000003d054.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_4.3510000003d054.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19387485
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1376160.7098187310009524TruSeq Adapter, Index 4 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG652860.33674300715126276No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG402710.20771647276580743No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC358940.1851400529774749No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG325750.16802076184714007No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA282140.1455268695243349No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT205160.10582084267247659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG168600.040.71243311
GTATGCC164200.040.5592645
CGTATGC166500.039.9575244
AGCACAC176200.039.12237510
ACGTCTG177150.038.8658915
ACTGACC173050.038.6284332
CTCGTAT173800.038.46084242
CACACGT179850.038.1782912
ACACGTC180650.038.06109613
CACGTCT187050.036.8323214
CGTCTGA188650.036.64005316
GAGCACA188750.036.61629
TCGTATG183300.036.3689443
AGAGCAC190500.036.268028
TCTCGTA186250.035.91554641
AGTCACT186900.035.8974128
CAGTCAC188950.035.61483427
CACTGAC189750.035.47772631
CTGAACT197100.034.95534519
CTGACCA191200.034.9040733