FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_3.3510000003cff8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_3.3510000003cff8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16805189
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC1510230.8986688575772638TruSeq Adapter, Index 3 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG587490.3495884515193492No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG356010.21184528183527124No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC301860.17962309141539556No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG291040.17318460387443427No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA278140.16550840338659684No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT183610.1092579202768859No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA179880.10703836773272826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC179700.041.93054245
CGTATGC182200.041.4289344
GCACACG186350.040.98790411
TAGGCAT182650.040.65735235
ACTTAGG185450.040.329232
CTCGTAT187000.040.30547742
AGCACAC192650.039.67088310
CACTTAG188300.039.64710231
CACACGT194550.039.31814212
ACGTCTG195100.039.25716815
TTAGGCA189200.039.23628634
ACACGTC195450.039.15023813
AGGCATC192050.038.76109336
CATCTCG193100.038.73355539
GAGCACA199550.038.2868549
CGTCTGA200150.038.24418316
TCGTATG198050.038.10188343
TCTCGTA197850.038.0971841
GGCATCT198000.037.8574837
CAGTCAC197750.037.7398527