FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_2.3510000003cf9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_2.3510000003cf9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16558916
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC738570.4460255731715772TruSeq Adapter, Index 2 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG627430.37890765313381625No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG373810.22574545338595836No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG319070.19268773390721952No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC312370.18864157533017256No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA308660.186401090506166No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT203060.12262880009778418No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC170820.10315892658674035No Hit
CCTCGATCTCGAACTCGTGGCCGTTCACGGAGCCCTCCATGCGCACCTTGA169180.10216852359176169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG99100.038.5486811
GTATGCC95750.038.32472245
CGTATGC97150.037.79490744
ACGTCTG104400.036.4447115
AGCACAC106250.036.01788710
CTCGTAT103550.035.52446442
CACACGT107550.035.3944712
ACACGTC108000.035.33138713
TATCTCG104700.035.1181939
CGATGTA105900.034.69879534
CGTCTGA110800.034.33970316
CACGTCT111600.034.2540114
AGAGCAC114900.033.4040578
GAGCACA114450.033.3191689
TCGTATG111600.033.12297443
CCGATGT112300.033.0588533
CAGTCAC114150.032.5799427
TCTCGTA113250.032.4242141
GATGTAT115000.032.15048235
ATGTATC115400.031.82444236