FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_12.3510000003d300.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_12.3510000003d300.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19417857
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC1237400.6372484873073274TruSeq Adapter, Index 12 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG544580.28045319316132566No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG394340.20308111240081744No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG323810.16675887560609803No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA271840.1399948511311006No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC236700.12189810646973043No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT233490.12024498892951986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG153350.041.19639611
GTATGCC153450.040.67430545
CGTATGC154950.040.39568344
ACGTCTG160950.039.21302415
AGCACAC161350.039.1677610
CTCGTAT160550.039.09898842
ACACGTC164550.038.40774513
CACACGT165150.038.29377712
CGTCTGA170850.036.99357616
CACGTCT173150.036.50161714
GAGCACA173600.036.364939
AGAGCAC174450.036.265128
TCTCGTA173250.036.26098341
CAGTCAC173150.035.86340727
AGTCACC174350.035.73307828
TCGTATG176900.035.5232443
CTGAACT179100.035.30244419
TCGGAAG185550.034.1262443
CCAGTCA189000.033.10614426
TGTAATC189000.032.95554436