FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_11.3510000003d295.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_11.3510000003d295.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18434584
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1119330.6071902680310008TruSeq Adapter, Index 11 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG433240.23501479610280332No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG330110.17907103301056318No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG273310.1482593803038897No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA205370.1114047379642524No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT195890.10626222973081464No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC189590.10284474008201107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG142600.041.06796611
GTATGCC143200.039.67193645
CGTATGC143300.039.6585544
ACGGCTA145000.039.15001732
AGCACAC150250.038.91687810
ACGTCTG150200.038.90462515
CGGCTAC146500.038.8266933
AGTCACG148000.038.5685428
CACACGT151850.038.55147612
ACACGTC152300.038.46909713
CACGGCT149500.038.0770431
CTCGTAT149400.037.8586942
GGCTACA149750.037.3997834
GCTACAT150850.037.08462535
CGTCTGA158750.036.9796716
CACGTCT159100.036.76974514
GAGCACA160750.036.556229
AGAGCAC160650.036.4109278
CATCTCG155550.035.9754439
CTACATC156650.035.71155536