FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_10.3510000003d252.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_10.3510000003d252.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17012764
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC780250.4586262408624489TruSeq Adapter, Index 10 (100% over 51bp)
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG444430.2612332716776651No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG321170.18878178760370742No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG301680.1773256832340706No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA197130.11587182423737846No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC186080.109376700928785No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT183690.10797187335344215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG96750.040.08915311
CGTATGC99150.038.4169344
GTATGCC100200.037.9477845
AGCACAC103850.037.4565510
ACGTCTG105700.037.01784515
CTCGTAT103700.036.66656542
CACACGT106150.036.51790612
TATCTCG105900.035.8660439
ACACGTC108350.035.8193213
TAGCTTA105850.035.62914334
CACTAGC107250.035.30770531
CGTCTGA111000.035.18951416
ACTAGCT108650.034.79062332
GTCACTA109550.034.46271529
GAGCACA113200.034.441949
CACGTCT114000.034.44039514
AGAGCAC115900.033.3290028
AGTCACT114900.033.0342928
TCGTATG116300.032.77108443
CAGTCAC116050.032.7067427