FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l03n01_empty_vector_1.3510000003cf58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l03n01_empty_vector_1.3510000003cf58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16769326
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG541090.3226665162332702No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG341910.20389012653221722No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG295820.17640542022976952No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC290040.1729586508127995No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA284420.16960729369802935No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT172640.1029498740736509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT62700.023.4712491
CTGACTC78850.018.6346232
ACTCTAG83450.018.2002015
CTAGCAG82250.017.7792228
GCACACG23700.017.56169711
GACTCTA89350.017.4521064
CGTATGC21100.016.1010144
TGACTCT97300.016.0030363
CTTCCGA68800.015.6665381
GTATGCC21350.015.38589145
TAGCAGA97550.015.1982469
CTCTAGC103300.014.8314446
ACGTCTG28850.014.81809315
CCGATCT75300.014.0148044
CCGGGGT10150.013.9669151
AGCACAC31650.013.86125910
CTATCGA106750.013.51048217
TCTATCG109600.013.20041416
CACACGT32350.013.14409312
CTCGTAT26050.012.52337842
Produced by FastQC (version 0.11.2)