FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_60_mchx_pn.3510000003d31d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_60_mchx_pn.3510000003d31d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13412908
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1555141.1594353737459469TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG188500.042.8117311
CGTATGC186000.042.1464944
ACGTCTG191700.042.11054615
CACACGT191800.042.08765412
CACGGCT188950.042.08702531
GTATGCC187150.042.06737545
ACACGTC194250.041.5568213
CGTCTGA196800.041.01926816
AGTCACG193350.040.9193228
TCGGAAG198500.040.7263153
GAGCACA199150.040.6237959
GTCACGG195900.040.60492329
ACGGCTA195700.040.5211532
CGGCTAC196300.040.48779333
GCTACAT191300.040.4380635
AGCACAC201250.040.0657510
TCACGGC199450.039.85963430
TCTCGTA197550.039.82124741
CATCTCG195200.039.73520739
CTCGTAT198900.039.64147642