FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_5_mchx_pn.3510000003d26e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_5_mchx_pn.3510000003d26e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15545210
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC1739371.1189105840319944TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG201700.042.977511
ACGTCTG205350.042.3371115
CGTATGC203400.042.26883344
GTATGCC204950.042.12454645
ACACGTC206650.041.9488513
CACACGT207850.041.71748712
CGTCTGA208600.041.65592216
GTCACAC207600.041.1108729
GAGCACA214800.040.3979389
AGCACAC215300.040.1891810
TCGGAAG218200.040.0821653
TCTCGTA216900.039.8358641
ATCTCGT217300.039.44077340
TCGTATG220100.039.307343
CTCGTAT220300.039.2823442
ACTCCAG220900.038.85612523
CACACTT220850.038.84929731
CTGAACT224200.038.77642419
TCACACT222300.038.59477230
AGAGCAC229400.037.9151088
Produced by FastQC (version 0.11.2)