FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_30_mchx_pn.3510000003d2d8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_30_mchx_pn.3510000003d2d8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14334264
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC6169504.3040228643758764TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG703350.043.9245711
CACACGT705850.043.74076512
ACGTCTG706800.043.6674315
GTATGCC699150.043.65269545
ACACGTC708150.043.60187513
CGTCTGA705150.043.54625716
TCGGAAG715900.043.464093
CGTATGC701950.043.3962944
GAGCACA713100.043.368179
AGCACAC712700.043.2536110
CACTAGC702300.043.1328231
CTGAACT713200.043.06060419
TCTCGTA711200.042.86485341
AGTCACT707500.042.81808528
CTCGTAT713800.042.71187242
ACTAGCT711150.042.67619332
TCACTAG712500.042.63445730
TCGTATG716150.042.61152343
AGAGCAC725950.042.6098068
ACTCCAG714100.042.60846323