FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_180_mchx_pn.3510000003d37a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_180_mchx_pn.3510000003d37a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17461014
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC4781212.738220128567562TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG550500.043.7988311
ACGTCTG554700.043.5991115
GTATGCC549600.043.568445
ACACGTC555600.043.4299313
CGTATGC550250.043.40281344
CACACGT556150.043.3586612
CGTCTGA559250.043.1920916
AGTCACC554150.042.92681528
GAGCACA565400.042.7241829
TCGGAAG568600.042.6947443
AGCACAC565700.042.56232510
TCTCGTA561600.042.49115841
ATCTCGT563400.042.1748340
TCGTATG568200.042.1708843
CTGAACT572250.042.1395119
CTCGTAT567500.042.13266842
ACTCCAG567200.042.05792223
TGTAATC561100.041.9717136
AGAGCAC580100.041.6454128
GATCGGA589600.041.4420241