FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_10_mchx_pn.3510000003d288.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_10_mchx_pn.3510000003d288.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14385650
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC5083753.533903577523435TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC578450.043.83924545
GCACACG582100.043.79808411
ACGTCTG582950.043.63955715
CGTATGC581000.043.58145544
CACACGT585700.043.4566512
CGTCTGA585900.043.42751316
ACACGTC587000.043.35657513
CGATCAG578050.043.29842433
AGTCACG581650.043.2607528
TCACGAT584500.043.0692130
GTCACGA583000.043.02564629
CACGATC583350.042.96660631
TCTCGTA588750.042.9401741
AGCACAC592950.042.89011410
GAGCACA594650.042.8819
CTCGTAT591800.042.8329442
TCGGAAG598850.042.8215523
CTGAACT594650.042.7686919
TCGTATG595550.042.67961543
ATCTCGT590050.042.6724740