FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_input_0_mchx_pn.3510000003d201.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_input_0_mchx_pn.3510000003d201.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19094540
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC4169312.1835090030972206TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG475700.043.6432111
GTATGCC475750.043.26968445
ACGTCTG480400.043.2556215
CGTATGC476350.043.1213944
CACACGT483750.042.9172912
ACACGTC484200.042.88205313
CGTCTGA487450.042.6669416
AGTCACC478800.042.3479928
TCGGAAG493700.042.3348663
GAGCACA492550.042.1819429
AGCACAC491700.042.12674710
TCTCGTA491500.041.7947441
CTGAACT497800.041.72504419
CTCGTAT492700.041.7112142
TCGTATG493200.041.70400243
CATCTCG489600.041.68992239
CCAGATC489750.041.50223533
ACTCCAG495650.041.3109723
ACCAGAT493450.041.2016732
ATCTCGT499000.041.1121240
Produced by FastQC (version 0.11.2)