FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_60_mchx_pn.3510000003d189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_60_mchx_pn.3510000003d189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16259424
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1505300.9258015536097711TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG182100.042.27756511
CGTATGC181500.041.79068444
GTATGCC183250.041.48918545
ACGTCTG185700.041.40057415
CACAGTG184200.040.81416733
ACACGTC190000.040.49714713
CACACGT190100.040.48768212
CGTCTGA189150.040.39565316
AGCACAC193100.039.96204810
GTCACAC190500.039.71111729
GAGCACA194900.039.5698979
TCGGAAG197450.039.4278373
TCTCGTA194550.039.0237141
CTCGTAT195750.038.78448542
TCGTATG198100.038.44839543
CTGAACT200050.038.2608319
ACACAGT197900.037.97794332
CAGTGAT198150.037.90498435
ACTCCAG202050.037.73723
ATCTCGT201550.037.7011840
Produced by FastQC (version 0.11.2)