FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_5_mchx_pn.3510000003d047.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_5_mchx_pn.3510000003d047.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17125863
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC889940.5196468055361647TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG104800.040.74547211
CGTATGC106000.039.56707444
ACGTCTG109250.039.3146415
GTATGCC108200.038.80370345
CACACGT110800.038.5394912
ACACGTC112000.038.30735413
CGTCTGA112900.037.98382616
CCGATGT110850.037.8173733
AGCACAC114000.037.5950610
GAGCACA116100.037.0700659
AGTCACC112550.037.00657728
GTCACCG113500.036.79595629
ACCGATG113600.036.58625432
TCGGAAG119250.036.283653
TCACCGA115350.036.1082830
CGATGTA117800.035.31878734
CTCGTAT119750.035.17522442
TCTCGTA119900.035.03738441
CTGAACT123500.034.94188719
TCGTATG122100.034.7188643
Produced by FastQC (version 0.11.2)