FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_30_mchx_pn.3510000003d12c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_30_mchx_pn.3510000003d12c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16075644
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4630802.8806310963343056TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC546650.043.4659745
GCACACG561950.043.4463111
CGTATGC545650.043.4183444
ACGTCTG564500.043.22085615
CGTCTGA564900.043.12652616
ACACGTC567350.043.00953313
CACACGT568400.042.92612512
AGCACAC569550.042.7517510
GAGCACA573750.042.689759
AGTCACT553900.042.66682428
TCGGAAG575000.042.6609423
CTGAACT572800.042.6174819
TCTCGTA557200.042.5363441
ACTGACC555950.042.49502632
CTCGTAT561500.042.23864742
TCGTATG563050.042.16154543
ATCTCGT559000.042.10486640
TCTGAAC581500.041.94556818
ACTCCAG569150.041.94127323
CTGACCA559850.041.84866733