FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_180_mchx_pn.3510000003d1e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_180_mchx_pn.3510000003d1e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14332322
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1269870.88601832975843TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG156100.042.0990811
ACGTCTG158550.041.522115
CGTATGC156950.041.38919444
CACGCCA154150.041.28165431
GTATGCC159300.040.9616745
CGTCTGA162800.040.45195816
ACACGTC162800.040.42220313
CACACGT163050.040.30502712
AGCACAC163050.040.20745510
TCACGCC160950.039.99792530
GAGCACA164600.039.9655159
CGCCAAT159050.039.8690733
AGTCACG162050.039.6985128
TCGGAAG167250.039.4702383
TCTCGTA166100.039.08371741
ACGCCAA162350.038.90675432
CTCGTAT167500.038.8376542
GCCAATA164000.038.4736134
TCGTATG170800.038.1921443
GTCACGC168650.038.17175329
Produced by FastQC (version 0.11.2)