FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_10_mchx_pn.3510000003d0cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_10_mchx_pn.3510000003d0cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16355916
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC5542143.388462009709514TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC632000.043.8593745
CGTATGC633400.043.67033444
CACTTAG636200.043.20123731
TAGGCAT630300.043.18527635
CGTCTGA655500.043.1170416
ACGTCTG655700.043.0592815
TCTCGTA644100.042.9432141
GCACACG657900.042.88886611
CTCGTAT645700.042.83680342
AGTCACT644400.042.81056228
ACACGTC660350.042.72371313
CATCTCG642250.042.71484839
TCGTATG649750.042.64866643
TTAGGCA638700.042.6474634
CTGAACT661900.042.59424619
CACACGT663100.042.54313712
ATCTCGT649900.042.46133440
CAGTCAC653100.042.3056927
GAGCACA666600.042.2886129
AGCACAC665400.042.26006310