FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l02n01_nlp7_chip_0_mchx_pn.3510000003cfeb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l02n01_nlp7_chip_0_mchx_pn.3510000003cfeb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16620839
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC285390.1717061334870039TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC41700.032.69803244
GCACACG44300.032.14691511
GTATGCC43800.031.3356145
ACGTCTG46100.030.74716815
CGTCTGA50800.028.07960316
ACACGTC52000.027.64678813
CACACGT52600.027.24588212
AGCACAC53600.026.61115810
TCACGAT52500.025.3727235
CTCGTAT54450.025.1250242
GAGCACA57350.024.9887969
TCTCGTA54650.024.95072641
CACGATC54850.024.73742336
TCGGAAG58500.024.3849773
CATCACG55600.024.32396533
CTGAACT59550.023.76466619
TCGTATG59050.023.47233643
ATCTCGT57800.023.43488740
ACTCCAG63900.022.3227323
CAGTCAC61750.022.00829127
Produced by FastQC (version 0.11.2)