FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_pd_36.3510000003d0f4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_pd_36.3510000003d0f4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10430902
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG438200.42009789757395855No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG373810.35836785735308413No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG345950.3316587577948676No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA189820.18197850962457512No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC185300.1776452314478652No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT149710.143525459255585No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG139390.13363177987867203No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT132210.12674838666876556No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC122350.11729570462842044No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC122020.11697933697392612No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT113900.10919477529364191No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108660.10417124041621711No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC106460.10206212271958838No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC106130.10174575506509409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT73500.022.3474331
ACTCTAG83650.019.6081715
GACTCTA85500.019.1580434
CTGACTC87450.018.9112262
CTAGCAG89450.018.3868058
CTCTAGC92500.017.6589186
TGACTCT97250.016.9585933
TAGCAGA100500.016.5438759
GCACACG8350.015.08856411
CTATCGA110650.014.864696517
TCTATCG112650.014.62075916
GGGGGAC5050.014.2577411
ATCTATC117550.013.97302115
GATCTAT119150.013.91690614
GCAGATC119900.013.88541511
CCGGGGT5200.013.846461
AGCAGAT120650.013.76219910
GCGGGCT6100.013.6478431
GGGGGAT9900.013.6366661
GCGGGGA13450.013.5504861
Produced by FastQC (version 0.11.2)