FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_pd_35.3510000003d097.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_pd_35.3510000003d097.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10913991
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG476310.43642147038603935No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG392940.3600332820505349No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG358270.32826671746384983No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA202600.18563328483595049No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC199050.18238057920333633No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT168700.15457223668225492No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG156680.14355885028675577No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT149460.13694348840859405No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC139360.1276893118200299No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC134650.12337375026239256No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134240.12299808566820332No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC123210.11289179182940504No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT122610.11234203876473786No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC118020.10813642782003394No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG117990.10810894016680059No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT113950.10440726953137491No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC110710.10143860298217214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT77550.022.3118521
ACTCTAG89000.019.8953655
GACTCTA90950.019.2963434
CTGACTC90500.019.0444872
CTAGCAG95250.018.5187768
CTCTAGC99000.017.7947436
TGACTCT99400.017.5196633
CCGGGGT5800.017.0693021
TAGCAGA106050.016.844699
GCGGGGA12950.016.6798651
GCGGGCT5300.016.5569291
TCTATCG119500.014.62972616
CTATCGA118700.014.614593517
ATCTATC124550.014.036550515
GCAGATC127100.013.91272911
GATCTAT126550.013.86735714
AGCAGAT128100.013.8573810
GGGGGGT5850.013.8464261
AGATCTA131450.013.41852513
TATCGAT133300.013.09829218
Produced by FastQC (version 0.11.2)