FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_pd_34.3510000003d07d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_pd_34.3510000003d07d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12800359
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG662710.5177276668568437No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG571500.4464718528597518No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG484980.3788799986000393No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC285730.22322030186809602No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA282780.2209156790055654No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT235820.18422920794643338No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG219610.17156550062384968No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT211650.16534692503546192No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC195820.15298008438669572No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC190100.14851145971765323No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC168930.13197286107366207No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT167490.13084789262551152No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC167190.13061352419881347No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG155190.12123878713089219No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT152870.1194263379644274No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC149250.11659829228227116No Hit
CTCGAAGTTCATCACGCGCTCCCACTTGAAGCCCTCGGGGAAGGACAGCTT146650.1145670992508882No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145060.11332494658938862No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT143280.1119343605909803No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCG139760.10918443771772339No Hit
CGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGACCCAGGACTCC136630.10673919379917392No Hit
GTGGTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTC135800.10609077448530935No Hit
AGCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAAT130510.10195807789453405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT101150.022.8890951
ACTCTAG115300.020.001235
CTGACTC117500.019.5504512
GACTCTA118150.019.538114
CTAGCAG123850.018.5112298
CTCTAGC127550.018.1506426
TGACTCT127700.018.1117743
CCGGGGT7450.017.214631
TAGCAGA135150.017.0631079
GCGGGCC3500.016.7141551
GCGGGCT6550.015.1143871
CTATCGA153400.014.91740817
TCTATCG156550.014.61725116
AGCAGAT158950.014.43743110
GCAGATC160600.014.28871211
GGGGGGT7100.014.2604511
ATCTATC162600.014.14256315
GCGGGGA17900.014.0781021
AGATCTA165050.014.02636113
GATCTAT163950.014.01140214
Produced by FastQC (version 0.11.2)