FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_md_33.3510000003d003.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_md_33.3510000003d003.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12187059
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG508750.41745100273987357No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG441840.36254850329353455No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG377600.3098368523529754No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC225180.1847697627458766No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA220310.18077372071473521No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT175180.14374263716947625No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG160500.13169707310024512No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT153610.12604353519581715No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC149490.12266290004832175No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC141810.11636113355978665No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132360.10860700682584698No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC129350.10613717386614768No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT125240.10276474414376757No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC122630.10062312818867947No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT78750.022.6007141
ACTCTAG94100.019.2470915
CTGACTC93650.019.1727432
GACTCTA95550.018.7203884
CTAGCAG97550.018.5892248
CTCTAGC103350.017.4808336
GCCGGGG8850.017.0344371
TGACTCT106000.016.8957213
TAGCAGA110450.016.5807849
CCGGGGT7000.015.1076211
GCGGGGG13700.014.617251
GCGGGGA15450.014.5635681
CTATCGA124650.014.42283917
GCGGGCC4100.014.2687441
GGGGGAT12650.014.2296991
GGGGGAC7200.014.0629451
TCTATCG129450.013.92280416
GCAGATC133100.013.75873411
AGCAGAT133950.013.65507910
GATCTAT131600.013.60890414
Produced by FastQC (version 0.11.2)