FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_md_32.3510000003cfb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_md_32.3510000003cfb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15243128
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG762300.5000942063859859No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG636670.4176767393149227No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG529220.34718595815766945No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA356050.2335806666453237No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC298260.19566850058596896No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC215770.14155231131038196No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT207530.1361465966827806No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT200720.13167900971506635No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG196090.12864157540368354No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT179120.11750868981747054No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC174990.11479927217038392No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT173450.11378898084435163No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC165340.10846855054946727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT105200.024.8116361
ACTCTAG124850.021.1200645
GACTCTA126950.020.7013684
CTGACTC126550.020.6251032
CTAGCAG132400.019.6947368
CTCTAGC141750.018.64966
TGACTCT144500.018.155683
TAGCAGA149750.017.6680859
GCGGGCT8750.016.7154641
CTATCGA174300.014.93592317
TCTATCG176950.014.66137716
GCGGGGA21150.014.5754971
GCAGATC181200.014.514276511
GGGGGAC8600.014.3905491
AGCAGAT185300.014.27844510
ATCTATC184000.014.20968115
GATCTAT185650.014.04647614
AGATCTA191700.013.76667913
GGGGGGT8550.013.6851761
TATCGAT197400.013.2678918
Produced by FastQC (version 0.11.2)