FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_pchx_pn_md_31.3510000003cf71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_pchx_pn_md_31.3510000003cf71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16552936
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG843030.509293336239565No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG706090.4265648100131602No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG597530.3609812784873934No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA393150.23751073525566704No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC335730.20282202504739943No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT243730.14724276104251233No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC238710.14421006641963696No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT231640.13993892080534837No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG226180.13664041231114527No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT196020.11842007967649969No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT195280.11797302907472125No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC195150.11789449315819261No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC187750.11342398714040822No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG173820.1050085616231465No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT169040.10212085638463171No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC166690.10070116866276774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT116200.025.1340521
ACTCTAG135850.021.7783325
CTGACTC138250.021.1902392
GACTCTA138500.021.1677884
CTAGCAG146450.020.0483978
CTCTAGC152650.019.4699366
TGACTCT153800.019.0469883
TAGCAGA161300.018.2440019
GCGGGCT8700.016.8107871
CTATCGA190450.015.34704417
TCTATCG192750.015.14056916
AGCAGAT202450.014.65796710
GCAGATC200700.014.65086111
GCGGGGA21400.014.4045851
ATCTATC204000.014.34972915
GATCTAT204300.014.34968714
GCCGGGG13000.014.1926811
AGATCTA209950.014.0380713
GGGGGAT16350.013.8994481
TATCGAT213100.013.86365918
Produced by FastQC (version 0.11.2)