FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_pd_30.3510000003cf4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_pd_30.3510000003cf4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11891842
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG576650.4849122617000798No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG486890.40943194502584207No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG382180.321379984698754No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA259520.21823364286205618No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC228320.19199716915175968No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT161960.13619420776024438No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC155780.13099736777532026No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG150990.1269693963306946No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT150540.12659098565218072No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT139760.1175259476202257No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC130520.10975591502140711No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC128160.10777136124075648No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC127260.1070145398837287No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT126410.1062997641576469No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT124420.10462634804599659No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG123330.10370975329137404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT84100.021.5365181
ACTCTAG88600.020.2640615
CTGACTC91600.019.7237832
GACTCTA93700.019.257644
CTAGCAG98000.018.3890868
CTCTAGC104450.017.4473846
TGACTCT105350.017.3199773
TAGCAGA107900.016.8891649
GCGGGGA14800.015.8105591
GCCGGGG7550.015.7944521
CCGGGGT6150.015.7314581
CGTATGC9150.014.75305944
CGCGGGG4250.014.293891
CTATCGA128150.013.94070717
TCTATCG128800.013.905292516
GATCTAT133750.013.4909214
GGGGGGT7750.013.3546271
AGATCTA136950.013.30661413
GGGGGAC7650.013.2350841
AGCAGAT139200.013.1723410
Produced by FastQC (version 0.11.2)