FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_pd_29.3510000003cf21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_pd_29.3510000003cf21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13067759
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG633070.4844518482472779No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG547020.4186027611926421No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG440310.33694377130768943No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA281040.2150636539899458No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC248030.18980301060036384No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT194500.1488395982815416No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG177650.13594526804481166No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT177470.13580752445771307No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC175520.13431530226414493No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC149970.11476336531764934No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC149090.1140899522251673No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT147410.11280434541224703No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT142240.10884804349391507No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC137260.10503713758418716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT93600.023.1742381
ACTCTAG104350.020.9357195
CTGACTC104850.020.6444172
GACTCTA107200.020.3173274
CTAGCAG116250.018.7923328
CTCTAGC120100.018.2461836
TGACTCT123000.017.688763
TAGCAGA127550.017.2329739
CGCGGGG4800.016.4070721
GCGGGGA16700.015.7642621
CCGGGGT7300.015.4117311
TCTATCG143200.015.03695416
CTATCGA144000.014.95341717
GATCTAT148750.014.62655914
AGATCTA151800.014.42089313
GCAGATC155050.014.21959111
ATCTATC152800.014.21002815
GCCGGGG10700.014.0894911
TATCGAT155200.013.93229518
AGCAGAT160150.013.72504310
Produced by FastQC (version 0.11.2)