FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_pd_28.3510000003cef9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_pd_28.3510000003cef9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973588
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG705720.50503850550052No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG611110.43733220129289635No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG485420.3473839360370436No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA303770.21738869072138092No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC286160.2047863440656759No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT218180.15613742154126772No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG208220.1490096888501364No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT196170.14038627731116732No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC187200.13396702407427497No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC173210.12395527905932249No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCC165140.11818009805355646No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT162430.11624072500205389No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGAT156480.11198269191849654No Hit
GGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTC153500.10985009719765602No Hit
GCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTC148070.10596419473652723No Hit
GCCAAATGTTTGAACGATCGGGAATTGGATCCGGTACCGAGCTCGAATTCT143320.10256492462780496No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGG141190.10104062034747269No Hit
GCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCG140980.10089033682687654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT104950.023.0034941
ACTCTAG112900.021.4625435
CTGACTC115850.020.9367162
GACTCTA116300.020.8940984
CTAGCAG127150.019.092378
CTCTAGC133100.018.2726866
TGACTCT134550.018.2436333
TAGCAGA139000.017.5613259
GCGGGGC6750.016.6664371
CGCGGGG5000.016.6497691
GCGGGGA17400.016.5514951
CTATCGA160150.015.00506717
TCTATCG161900.014.80118116
GGGGGAT16950.014.6015671
GATCTAT167350.014.41255414
AGATCTA169300.014.33875613
GCAGATC172550.014.09425511
ATCTATC171350.014.03741715
AGCAGAT176000.013.94615110
TATCGAT172950.013.93357418
Produced by FastQC (version 0.11.2)