FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_md_27.3510000003ceb5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_md_27.3510000003ceb5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13396795
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG491970.36722962469754894No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG424080.31655332488106297No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG389840.29099497305139027No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC199250.14872960286396858No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA198630.14826680560537053No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT171610.12809780249679123No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT159230.11885678626865603No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154470.11530369763812912No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG150670.11246719831123786No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGAT141490.10561481309522165No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC140320.10474146988141567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT87750.022.0769841
ACTCTAG97400.019.7270245
CTGACTC99950.019.3598792
GACTCTA101500.018.9752084
GCCGGGG10300.018.7864631
CTAGCAG107150.017.8479278
CTCTAGC112800.017.1932986
TGACTCT114750.017.1761343
TAGCAGA119050.016.2712849
CGCGGGG4900.014.6939191
TCTATCG134200.014.20123116
CTATCGA134100.014.17826317
GCGGGGA17350.014.1354861
GCGGGGC6300.013.9286111
GATCTAT142250.013.42835914
TATCGAT142850.013.388556518
GCAGATC143700.013.33893611
AGCAGAT146950.013.2585610
AGATCTA145200.013.2015813
ATCTATC143950.013.19246315
Produced by FastQC (version 0.11.2)