FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_md_26.3510000003ce8f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_md_26.3510000003ce8f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16409954
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG609450.3713904377794112No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG537890.32778275917165883No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG438190.26702695205605087No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA278840.16992125633015182No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC265470.16177376243711591No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT226410.1379711362993461No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT220720.13450372865152457No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG186410.11359568710552143No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC176380.1074835432201699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT94350.022.0355931
ACTCTAG103050.020.0861245
CTGACTC104500.019.6367932
GACTCTA109200.018.8525714
CTAGCAG117650.017.7463238
GCGGGGC7500.017.4004881
GCGGGGG24850.017.2942471
CTCTAGC122000.017.2610956
CGCGGGG7550.016.987231
TGACTCT125850.016.53693
TAGCAGA130100.015.9959549
GCCGGGG14350.015.2094851
GGGGGAT20650.014.8188171
CTATCGA144300.014.19001917
GCGGGCC6700.014.1048721
TCTATCG145850.013.96207716
GCGGGCT12200.013.4634911
GCGGGGA22550.013.3706621
AGATCTA155450.013.27183313
TATCGAT154650.013.19669718
Produced by FastQC (version 0.11.2)