FastQCFastQC Report
Mon 16 Nov 2015
C7KGBACXX_l01n01_nlp7_mchx_pn_md_25.3510000003ce65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KGBACXX_l01n01_nlp7_mchx_pn_md_25.3510000003ce65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16340442
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACG651400.3986428274094422No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGG578190.35383987777074816No Hit
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATG519140.3177025443987378No Hit
CGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGAC282080.17262690935777625No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGA277120.16959149575023735No Hit
AGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCT235670.1442249848565908No Hit
GGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATG228020.13954334894980197No Hit
CAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC206060.1261042999938435No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGAC203470.12451927554958428No Hit
CGGGGATGTCGGCGGGGTGCTTCACGTAGGCCTTGGAGCCGTACTGGAACT200090.12245078805089851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT112500.020.700331
ACTCTAG121350.019.486025
CTGACTC125550.018.6023332
GACTCTA128100.018.6004854
CTAGCAG136000.017.519168
GCGGGGC8400.016.8752691
CTCTAGC142700.016.5705246
TGACTCT146150.016.2568323
TAGCAGA152250.015.8706699
CGCGGGG6750.015.3335771
GCCGGGG12900.015.1746611
CTATCGA171300.013.71304517
GGGGGGA11350.013.6786311
GCGGGGA24050.013.6592631
TCTATCG172700.013.64096516
AGATCTA180950.013.19178913
GCAGATC181200.013.0988111
ATCTATC180600.013.04426815
GATCTAT181750.012.99811514
GGGGGGT9750.012.9232831
Produced by FastQC (version 0.11.2)