FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l08n01_151022_gms8.351000000381bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l08n01_151022_gms8.351000000381bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155819075
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG1585717510.17665840976145Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT141980119.111856812139335Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA140800469.036150419966233Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC113315017.272216832245988Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA91097365.846354818882092Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC31713532.035279056816375No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT29158241.8712882232165735Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT19655581.2614360597378722Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC17172651.1020890734975803Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC16275271.0444979217082377Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA15401480.9884207052313717Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG12421640.7971835283966355Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC10622740.681735532058575No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA9924540.6369271541369373Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA9009570.5782071290052261Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT8321010.534017417315563Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT7977060.5119437398790874Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA6814070.4373065364429868Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTC5600520.3594245441387712Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA5448690.34968055098517303Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG5008800.3214497326466609Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG4861780.31201443083909974Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC4328670.2778010330250003Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA4268590.2739452791643128Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT4163860.2672240224760672No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA4128480.26495344039232677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA4107210.2635883957082918Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTG4105260.2634632505680065Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA3983010.25561761292704377No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT3981950.2555495853123246No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA3634900.23327695919129285Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA3572900.22929798550017064Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAAC3556720.22825960172077778Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC3552640.22799775958110394Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTC3537510.22702676164648006Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC3284340.21077907181774758No Hit
GCCAAGGACGTTTTCATTAATCAGATCGGAAGAGCACACGTCTGAACTCCA3068730.19694186992189497Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCC3029080.19439725206942732Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTTTGGTGGCAGGATAAATCCATAGGAGATCGGAAGAGCACACGTCTGA3026800.19425092852078604Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT3023520.1940404279771267Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCCAGCATCAGTTTTGGTGGCAGGATAGATCGGAAGAGCACACGTCTG3016400.19358348777259782Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCAAGGACGTTTTCATTAATCAAGAACGAAGATCGGAAGAGCACACGTCT2801330.17978094145405496Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAACTCC2763400.17734670803301844Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC2711120.17399153473347215Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA2562800.1644728028323875Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAA2479180.15910632250897397Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGAGCATAGCTGTTGGGACCCGAAAAGATCGGAAGAGCACACGTCTGAAC2477990.1590299518849024Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCTG2472770.15869494797090794Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCTGA2458520.1577804257919Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTG2440820.15664449298007962Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACT2403260.15423400504719978Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA2402330.15417432044183293Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGA2395320.15372443970675606Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTC2316290.1486525317904756Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGAATCATCGAATCTTTGAACGCACAGATCGGAAGAGCACACGTCTGAAC2239160.14370256016472951Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATGGATACGAATAAGGCGTCAGATCGGAAGAGCACACGTCTGAACTCCA2138880.13726689110431442Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTACGATCTGCTGAGATTAAGCCTTTGTAGATCGGAAGAGCACACGTCTGA2136990.1371455965837302Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC2136920.13714110419407893Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG2054980.13188244122229578Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG2052780.131741251833256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGATCTGCTGAGATTAAGCCTTTGTTAGATCGGAAGAGCACACGTCTGAA2010050.12899896883613254Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTGA1986420.12748246644385483Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC1975970.1268118168459157No Hit
ATGATTAATAGGGACGGTCGGGGGCATCAGATCGGAAGAGCACACGTCTGA1945140.12483324008950765Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTTTTGGTGGCAGGATAAATCCAGATCGGAAGAGCACACGTCTGAACTCC1829650.11742143893486724Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACACGTCTGAA1810750.11620849372902516Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAAC1798240.11540563952134872Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTTGGACGTGGGTTAGTCGATCCTAAGAGATCGGAAGAGCACACGTCTGA1753170.11251318235588294Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC1728670.11094084597793948No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGATCGGAAGAGCACACGTCTGAACTC1670560.10721152079743766Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAA1642440.1054068636975287Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACGTCT1607500.10316451949159626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG1595340.10238412723217616No Hit
TGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGAA1593800.10228529465984829Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATCTGCTGAGATTAAGCCTTTAGATCGGAAGAGCACACGTCTGAACTC1591960.10216720898901498Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG1556500.044.1413881
TAGCCTG2034350.043.917941
ATTCCAG3078300.043.8236731
TCAGTCT115850.043.13311
GGTACAC324350.042.4219062
TGGGCCA608450.042.30241
TCGTACA38750.042.113371
CGGTACA140700.042.058151
CGTACAC97000.041.754992
CAGTCTC120350.041.412862
GAGCTAC125350.040.6944122
CTATGTT912900.040.648621
TGCTCGA1693400.040.5857852
GTCTAGT39750.040.4310261
TGCGACT45950.040.217151
TACACGG849700.040.207284
GTCTCAT124550.040.1922044
TAATCGC45400.040.14046510
GTCCTAT28000.039.9398082
GCGACTG46100.039.9263842
Produced by FastQC (version 0.11.2)