FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l07n01_151022_gms7.3510000003a4ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l07n01_151022_gms7.3510000003a4ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180942878
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT130623467.219044012331892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG117755626.507889191416531Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA103182775.702505185089407Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA61335633.3897786239478296Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC57801593.1944661563302867Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA29494041.630019392086822No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC26938851.4888041075592928No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG21523771.1895339699416079No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT21366461.180840066001382No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC17884160.98838706434193No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC17217340.9515345500362827No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG12953820.715906596776912Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT11800050.6521422744254128Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT11474770.6341653303425404Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG11263870.6225097182327343No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC11261180.6223610525306223No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT8506860.47014063742260137No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT8287060.45799315737644014No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT8267610.4569182325042934No Hit
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC7017990.3878566582764313Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC6989600.386287654825519Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA6943050.38371501972020144Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC6746780.3728679500720664No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA6654390.36776191876421904Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT6569900.36309248933246213Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT6334720.35009501727943115No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA5963270.3295664391941417Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC5919600.32715297034238616No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG5382820.29748725451354874No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA4718260.2607596415040994Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA4676480.25845062550624404Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCGATATAGTGTAACGGCTATCACATCAGATCGGAAGAGCACACGTCTGA4480680.24762953090643336Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT4285090.23682004217927827No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC4154600.22960837397534928No Hit
GCCAAGGACGTTTTCATTAATCAAGAACAGATCGGAAGAGCACACGTCTGA4113510.22733749155907645Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTC3397030.1877404647006886No Hit
TGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCT3121560.1725163230795964Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC3043890.16822380817884416No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT3005240.166087774949617No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC2978220.1645944860012672No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC2941780.16258059076522483No Hit
GCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGTCT2826390.15620344007129144Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACG2556160.14126889260598585No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC2530070.13982700109368218No Hit
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG2521610.13935945022384358Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA2471270.13657735675012309No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA2364920.13069981124098182Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC2321390.12829407963766332No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT2300150.127120228517643Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC2176580.12029100145074514Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG2066690.11421781408826712Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AATTCTAGAGCTAATACATGCTAGATCGGAAGAGCACACGTCTGAACTCCA2012640.11123068353096495Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGCTACCAACTGTAGAAAGAGAAAGTGAGATCGGAAGAGCACACGTCTG1989170.10993358909655455Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT1974470.10912117801066479Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA1910430.10558193951131914Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTTTGGTGGCAGGATAAATCCAAGATCGGAAGAGCACACGTCTGAACTC1873290.10352935803309153Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTCTG1867490.10320881488355678Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTCG3186000.044.014141
TCAGTCT533900.043.31271
ATTCCAG7141500.042.6634831
ATAGCCT473900.041.9232641
CAGTCTC554400.041.785212
TGCTCGA3381050.041.482542
GTCTCAT570450.040.947084
TAGCCTG5027950.040.643981
GCTCGAA3452950.040.6211363
CTCGAAT3518800.040.404894
CATAATC585650.040.0185178
TCTCATA584200.039.948245
CGATATA1694000.039.7907333
ATATAGT1701500.039.779425
CCGATAT1710000.039.629592
ATATATT3618200.039.558259
AATATAT3621950.039.5185438
GTCCTAT172300.039.4572752
GAATATA3628350.039.4488337
CTCATAA596700.039.318896
Produced by FastQC (version 0.11.2)