FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l06n01_151022_gms6.351000000381a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l06n01_151022_gms6.351000000381a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190040084
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT2180379611.473261609377104Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG169268648.906996694444738Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA139183667.323910675602521Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA84047224.422604864771581No Hit
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC74904413.9415058351584396Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA73357333.8600977465364625Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG54665832.876542087826061No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC43134412.269753259001927No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC34575051.8193556470960093No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC32234251.6961816329232942No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT27103381.4261928025668522No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG22680261.193446115294287No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT15603560.8210667808376679No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC14126830.7433605428210609No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT12728140.6697608068832468Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC10960220.5767320119685908No Hit
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG10495250.5522650684578734No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGT10199920.536724662782195No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT9936470.5228617979352188No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG9460730.4978281318797986Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA9176330.482862868025253No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT8025160.422287752724841No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT7846050.41286289896609385Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC7084460.3727876693634802No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGATCGGAAGAGCACACGTC6950650.365746523244012No Hit
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC5973100.3143073752798383Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TATCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA5763220.30326338942262304No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC5653850.2975082877778564No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGATCGGAAGAGCACACGTC5633980.29646271888619036No Hit
TGTAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT5422550.28533717128855823Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC5124580.2696578475517828Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCAC5097900.2682539332070596No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA4331380.2279192846494427Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA4152280.21849495709547256No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT4095760.21552084769653124No Hit
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT4030110.21206631333629591No Hit
TTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCAC3950160.20785930614511833No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT3852680.2027298619800652Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG3747790.197210500075342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGAGATCGGAAGAGCACACG3337100.17559979609354415No Hit
ATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACA3320140.174707352791951No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA3313200.17434216667679436Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT3310770.17421429891601184Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA3255490.17130543890940397Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACACG2920070.1536554782832026No Hit
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT2808700.14779513568305935Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCAGTCTCATAATCTGAAGGTCGAGAGTAGATCGGAAGAGCACACGTCTGA2750680.14474209556758563Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGT2741360.1442516727155309No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCAGATCGGAAGAGC2664910.14022883719626225No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA2637900.1388075580939019Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC2466700.12979893231366915No Hit
TATGGAATCTAAACCATAGTTATGACGATTGCTCTAGATCGGAAGAGCACA2448790.12885649955827214No Hit
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA2379680.12521989834523542Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCAAGGATGCTGGCATAATGGTTATATGCCAGATCGGAAGAGCACACGTC2258610.1188491371115159No Hit
TATCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC2212740.11643543579995472No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTAGATCGGAAGAGCACACGTC2173110.11435008626916834No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG2097860.11039039532312561Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATGGAATCTAAACCATAGTTATGACGATTGCTCTAGATCGGAAGAGCAC2022070.1064022893191312No Hit
TGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTG2018580.10621864385199915Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG1955750.10291249923884478No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAA1924160.10125021834867216Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACGATCAACTTAGAACTGGTACGGACAAGAGATCGGAAGAGCACACGTCTG1920260.10104499848568789Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC1903170.10014571452199526No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCAG10665450.044.479471
TTTCTCG317850.044.2532421
TTGCTCG4355750.044.185551
ATAGCCT1221000.044.081571
TATCCTG1116150.044.0540921
TCAGTCT310300.044.0170171
GCCAGGG686100.043.755593
TCTCGAA334300.043.6723943
ATCCTGT1126200.043.65222
TCCTGTG1158900.043.5586053
CAGTCTC314550.043.2541852
TAGTCTG112350.043.234971
TACTCGA91950.043.1469652
TTGCTCC461850.042.912641
TTACTCG92650.042.8072431
TTCTCGA328950.042.749432
TGCTCGA4553500.042.276992
TACCCTG130050.042.2660941
ATACCAG362850.042.1769071
TTTGATG1370100.042.1742441
Produced by FastQC (version 0.11.2)