FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l05n01_151022_gms5.35100000038189.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l05n01_151022_gms5.35100000038189.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125310832
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCT1348788010.763538781707235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTG110016428.779482048287733Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTC84170376.716926913389259No Hit
AAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGA72770955.807235403241117Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGT50272834.011850308359616No Hit
AAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAA36151432.884940545283428Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAAC30644012.4454398323682027Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTC29743982.3736160334487284No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACG16278631.2990600844466502No Hit
GGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACAC15454181.2332676875052588No Hit
AGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAGT15304441.2213182017656703Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACA12432850.9921608373009606No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGT11609660.926468990326391No Hit
ACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACAC11529700.9200880575112613No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGT10704180.8542102728996325No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTG9402660.7503469452664714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTCAGATCGGAAGAGCACACGTCTGA9208530.7348550682354419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCT9021240.7199090338814446Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACG7169030.5720997846379314No Hit
AAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACT6674130.5326059921140736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGT6489880.5179025545054238No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTG6371610.5084644238895485Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACG5987210.4777887038528321No Hit
TTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCA5723940.4567793468963641Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGA5475140.4369247185271262Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTG5431480.43344058237519323Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCC5321640.42467517891829176Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGCTCGAATATATTAGCATGGAATAATAGAATAGATCGGAAGAGCACACG5168620.4124639440587228No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGAA5041800.40234351009655733Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTC4799980.3830458966228873Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCAGGGGGCATGCCTGTTTGAGCGTAGATCGGAAGAGCACACGTCTGAA4413630.3522145635422802Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTC4401530.35124896465454797Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAGATCGGAAGAGCACAC4151570.33130176647458537No Hit
CTATGTTCCTTGGAACAGGACGTAGATCGGAAGAGCACACGTCTGAACTCC3233320.25802398311424507Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGAGGTGCACAATCGACCGATCCTGAAGATCGGAAGAGCACACGTCTGAAC2992070.23877185653032773Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACACGTCTGAACTCCAG2808750.2241426343733796Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACA2746830.21920132171814166No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTAGATCGGAAGAGCACACGTCTGA2737670.21847033941966007Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGAGGTGCACAATCGACCGATCCTGAGATCGGAAGAGCACACGTCTGAACT2652770.21169518689334055Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAAGATCGGAAGAGCACAC2421270.19322112552887688No Hit
CTATGTTCCTTGGAACAGGACGTCATAGATCGGAAGAGCACACGTCTGAAC2372410.18932202125990194Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTGCTCGAATATATTAGCATGGAATAGATCGGAAGAGCACACGTCTGAACT2367230.18890864917407937Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGATGATTCATAATAACTTTTCGAATCGCAAGATCGGAAGAGCACACGTC2276920.18170177020291428No Hit
AAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGAACTCCA2257810.18017676237278513Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATGCCTGTTTGAGCGTCATTTCCTTCTCAAACAGATCGGAAGAGCACACGT2038930.16270979670775787No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGATCGGAAGAGCACACGT2009640.16037240898695815No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATAGATCGGAAGAGCACACGTCT1969600.1571771544857351Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGCCTGTGGGAATACTGCCAGCTGGGACTAGATCGGAAGAGCACACGTCT1908480.15229968307927283Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTACAACGGGGTATTGTAAGCAGTAGAGTAGATCGGAAGAGCACACGTCTG1796940.14339861696872302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCT1776170.14174113854738432Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTCT1695970.13534105335762195Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATCGGAAGAGCACA1653840.13197901359397246No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGTAGATCGGAAGAGCACACG1608160.12833367828888087No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGAGTAGATCGGAAGAGCACACGTCT1511460.12061686734312002Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTCTGA1487860.11873355050423734Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAAAAAATTAGAGTGTTCAAAGAAGGCGTAGATCGGAAGAGCACACGTCTG1478790.11800975034624302Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTCTGA1452010.11587266454347697Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATACTGCCAGCTGGGACTGAGGACTGCGACGAGATCGGAAGAGCACACGTC1446210.11540981548985327No Hit
TTGCTCGAATATATTAGCATGGAATAATAGATCGGAAGAGCACACGTCTGA1402910.11195440789986935Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGAATCTTTGAACGCACATTGCGCCCCTAGATCGGAAGAGCACACGTCT1386500.11064486428435812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAAAATTAGAGTGTTAAAAGCAGGCGTAGATCGGAAGAGCACACGTCT1370570.10937362541811231Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTAGATAGGAAGAGCACACGTCT1316960.10509546373453174Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCTGAGATTAAGCCTTTGTTGTCTGATTTGTAGATCGGAAGAGCACACGTC1315920.10501247011112334No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGATCGGAAGAGCACACGTC1271340.10145491652309835No Hit
ATGTTCCTTGGAACAGGACGTCAGATCGGAAGAGCACACGTCTGAACTCCA1267400.1011404983728781Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCTG2362250.044.639121
ATTCCAG9491100.044.4619871
TTGCTCG1291600.044.267541
TCAGTCT88250.042.343831
TGCTCGA1351150.042.30812
GCTCGAA1376950.041.510683
CTATGTT1289250.041.4078561
CTCGAAT1385100.041.295864
TGGGCCA280750.040.9569741
CAGTCTC90250.040.945132
AATATAT1445800.040.5039988
GAATATA1447050.040.4920927
ATATATT1446500.040.490629
GTCCTAT30700.040.3914532
TCGAATA1446350.040.1068955
CGAATAT1450400.040.0689246
TATATTA1461150.040.00747710
ATATAGT163650.039.7870255
GTACAAC381250.039.7376331
GTTACGA359200.039.7140431
Produced by FastQC (version 0.11.2)