FastQCFastQC Report
Wed 28 Oct 2015
C7KCWACXX_l04n01_151022_gms4.3510000003a53f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC7KCWACXX_l04n01_151022_gms4.3510000003a53f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42354132
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGACAACAAAGGCTTAATCTCAGCAGATCGGAAGAGCACACGTCTGAA3018810.7127545430514313Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC742500.17530757093546387TruSeq Adapter, Index 11 (100% over 50bp)
GTCGACGTACTTCATAGGATCATTTCTATAAGATCGGAAGAGCACACGTCT524400.12381318545260236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAAGATCGGAAG445550.10519634778491033No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGT241300.036.9199682
GCTGTAA111050.036.046273
GGGAGAC457000.035.502671
CTGTAAT115350.034.8171124
TCATAGG255350.034.69634612
CGACGTA256250.034.631873
GGCTGTA119050.033.8342672
CATAGGA264150.033.5745313
ATAGGAT266350.033.3143414
CGGCTCG14000.032.30055226
GGATCAT275300.032.2401117
CGATATA92600.032.172143
TGTAATG126900.031.8946325
GACGTAC282250.031.6307774
TATAGTG106350.031.5842346
AACGGCT114200.031.42256414
GTAATGG128850.031.3420986
TTCATAG283700.031.2846711
TAGGATC286350.031.07456215
ACTTCAT290700.030.7019719
Produced by FastQC (version 0.11.2)